Haemacytometer

Title * Haemacytometer Objective * To perform manual cell reckon Apparatus & angstrom; Materials * Heamacytometer * ocellus sample (female) * Distilled water * Thoma red blood cell pipette * Microscope * Microscope slide * Tally counter Procedure * Blood is wasted to the 0.5 mark on Thoma RBC pipette * hence the diluting liquid to is drawn to the 101 mark * The mixture is therefore shake for 5 minutes * The c over glass is situated over the hemacytometer chamber. * With a Pasteur or transfer pipette, both put up of the hemacytometer are change (without overflow) by capillary action. Cells will slump in the furnish and in the pipet by dryness within a few seconds. * Worked quickly. * Using the microscope with a 10X optic (and a 10X objective), the cells in each of 10 squares (1 mm2 each) are counted. If over 10% of the cells understand clumps, paraphrase entire sequence. If fewe r than two hundred or more than than 500 cells are present in the 10 squares, repeat with a more suitable dilution factor. * The look of cells per ml is calculated, and the intact number of cells, in the original assimilation as follows: Cells/ml = average count per square x 104 perfect cells = cells per ml X any dilution factor X total pot of cell preparation from which the sample was taken. * calculation repeated to fleck reproducibility (+/- 15%).

Result square off| Cell counted| A| 90| B| 88| C| 81| D| 98| E| 79| Total| 436| * Calculation = Avg ×DFA mm2×D (0.1 mm ) = 436 ×2000.20 mm2× (0.1 mm ) = 436 ×10,000/?L =4,360,! 000 (or 4.36 ×106)/?L or 4.36 × 1012/L Discussion * Normal background grownup Male| 4.5-6.0 × 1012 / L| Adult Female| 4.0-5.5 × 1012 / L| newborn baby| 5.0-6.3 × 1012 / L| * Sources of error * Falsely high counts * Collection of promissory note of business from the area where there is hemoconcentration. * Inadequate wiping of the pipette. * Improper mixing. * unexpended distribution in...If you want to get a full essay, site it on our website: BestEssayCheap.com

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